10. Biodegradable Polymers for gene / nucleic acid Delivery SUNBRIGHT® ME-DT & DT Series

NOF has provided new biodegradable polymers for gene/nucleic acid delivery, SUNBRIGHT® ME-DT and DT Series. 
The poly cationic block polymer, PEG-PAsp(DET) (polyethylene glycol-b-poly{N'-[N-(2-aminoethyl)-2aminoethyl] aspartamide}), and homo polymer, PAsp(DET) forming polymeric micelles with polyanions such as gene/ nucleic acid (ex. plasmid DNA and mRNA) enable efficient delivery of gene/nucleic acid with a low toxicity compared to conventional cationic carriers.

 

 

Figure.1 Formation of polymer micelles through the electrostatic interaction between SUNBRIGHT® ME-DT (PEG-PAsp(DET) Block) / SUNBRIGHT® DT (PAsp(DET) Homo polymer) and plasmid DNA.

 

Figure.2 Protonation behavior and membrane disruption activity of SUNBRIGHT® ME-DT series (PEG-PAsp (DET) Block,).

 

Figure.3 Protonation behavior and membrane disruption activity of SUNBRIGHT® ME-DT series (PEG-PAsp (DET) Block,) K. Miyata et al. J. Am. Chem. Soc. 130 :16287-16294 (2008)

SUNBRIGHT® ME-DT, DT series having characteristic protonation behavior responing to endo-somal pH condition achieve selective disruption of endosomal membranes, follo-wed by efficient delivery of plasmid DNA and mRNA etc.

K. Miyata et al. J. Am. Chem. Soc. 130 :16287-16294 (2008) 

 

 

Examples of use for SUNBRIGHT® ME-DT and DT series >>

(PAsp(DET), homo polymer)
(DP of PAsp(DET)=ca. 50 ) *make-to-order


PEG-PAsp(DET), block polymer
MW of PEG=ca. 12,000, DP of PAsp(DET)=ca. 70 *make-to-order